HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kuhnt, K. Articles by Jahreis, G. Search for Related Content PubMed PubMed Citation Articles by Kuhnt, K. Articles by Jahreis, G. Agricola Articles by Kuhnt, K. Articles by Jahreis, G.

Dietary supplementation with 11trans- and 12trans-18:1 and oxidative stress in humans^1,2,3

¹ From the Institute of Nutrition, Friedrich Schiller University, Jena, Germany (KK, JK, and GJ), and the Division of Clinical Nutrition and Metabolism, Department of Public Health and Caring Sciences, Faculty of Medicine, Uppsala University, Uppsala, Sweden (SB)

Background: High consumption of trans fat has been associated with high oxidative stress in humans, which could increase the risk of the development or acceleration of several diseases, such as atherosclerosis, cancer, and type 2 diabetes.

Objective: Several urinary and blood biomarkers of oxidative stress [8-iso-prostaglandin-F₂ (PGF₂), 15-keto-dihydro-PGF₂, and 7,8-dihydro-8-oxo-2'-deoxy-guanosine in urine and -,ß-,-,-tocopherol, and retinol in plasma] were monitored to evaluate the oxidative stress induced by dietary supplementation of 11trans- and 12trans-18:1 isomers in humans during a 6-wk intervention.

Design: After a 14-d adaptation period free of trans fatty acid supplementation (baseline), the test group (n = 12) received 3.0 g 11trans-18:1/d and 3.0 g 12trans-18:1/d ( 6.0 g/d), and the control group (n = 12) consumed a control oil free of trans fatty acids and conjugated linoleic acids for 6 wk.

Results: The postintervention concentration of urinary 8-iso-PGF₂ (free radical–induced lipid peroxidation) in the test group was significantly higher than baseline and significantly higher than that observed in the control group. The concentrations of 15-keto-dihydro-PGF₂ (cyclooxygenase-mediated inflammatory response indicator) and 7,8-dihydro-8-oxo-2'-deoxy-guanosine (oxidative DNA damage) were not affected by the 11trans- and 12trans-18:1 supplementation.

Conclusions: Although an increase in urinary 8-iso-PGF₂ was observed and the effects of prolonged high (ie, >5.0 g/d) consumption of trans fat could be relevant to the development of disease, the mean intakes of 11trans- and 12trans-18:1 in Europeans are estimated to be significantly below the amounts administered in this study (ie, 6.0 g/d); such low intakes could minimize the possible risk of detrimental effects on human health.

Key Words: Oxidative stress • trans fatty acids • conjugated linoleic acids • isoprostanes • prostaglandins • 7,8-dihydro-8-oxo-2'-deoxyguanosine

This article has been cited by other articles:

				L. C. Vinikoor, J. C. Schroeder, R. C. Millikan, J. A. Satia, C. F. Martin, J. Ibrahim, J. A. Galanko, and R. S. Sandler Consumption of trans-Fatty Acid and Its Association with Colorectal Adenomas Am. J. Epidemiol., August 1, 2008; 168(3): 289 - 297. [Abstract] [Full Text] [PDF]

				A. Motard-Belanger, A. Charest, G. Grenier, P. Paquin, Y. Chouinard, S. Lemieux, P. Couture, and B. Lamarche Study of the effect of trans fatty acids from ruminants on blood lipids and other risk factors for cardiovascular disease Am. J. Clinical Nutrition, March 1, 2008; 87(3): 593 - 599. [Abstract] [Full Text] [PDF]