HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mariotti, F. Articles by Tomé, D. Search for Related Content PubMed PubMed Citation Articles by Mariotti, F. Articles by Tomé, D. Agricola Articles by Mariotti, F. Articles by Tomé, D.

Postprandial modulation of dietary and whole-body nitrogen utilization by carbohydrates in humans^1,2

¹ From the UMR INRA, Institut National Agronomique Paris-Grignon, Unité de Physiologie de la Nutrition et du Comportement Alimentaire, Paris, and the Service d'Hépato-Gastroentérologie, Hôpital Avicenne, Bobigny, France.

Background: Sucrose exerts a sparing effect on whole-body protein metabolism, mainly during the absorptive phase.

Objective: We aimed to characterize the acute postprandial effect of addition of sucrose on deamination of dietary and endogenous nitrogen, with particular consideration being given to the effects of bioavailability.

Design: Twenty-one subjects equipped with ileal tubes ingested ¹⁵N-labeled soy protein combined with [¹³C]glycine, with (n = 10) or without (n = 11) sucrose. Dietary and endogenous ileal flow of nitrogen were determined from the ileal effluents. The kinetics of dietary amino acid transfer to the blood were characterized by ¹³CO₂ enrichment in breath and ¹⁵N enrichment in plasma amino acids. Deamination of dietary and endogenous amino acid was determined from body urea, urinary nitrogen, and ¹⁵N enrichment.

Results: ¹³CO₂ recovery in breath and ¹⁵N plasma amino acid enrichments were highly correlated (R² 0.95, P < 0.001, for both meals) and markedly delayed by sucrose (half-¹³CO₂ recovery: 274 min compared with 167 min), whereas exogenous and endogenous ileal nitrogen kinetics and balances remained unchanged. Addition of sucrose halved the early (0–2 h) deamination peak of dietary nitrogen and reduced endogenous nitrogen oxidation over the first 4 h. Both were reduced by 18–24% over the 8-h period after the meal.

Conclusions: Without changing the nitrogen absorptive balance, sucrose markedly affected the bioavailability profile, which is governed by gastric emptying. Endogenous and dietary nitrogen were not spared in the same way and over the same periods, showing that the metabolism of endogenous and dietary nitrogen may be affected differently by nutritional modulation, even if the effects are of a similar magnitude over the entire postprandial period.

Key Words: Adults • nitrogen isotopes • carbon isotopes • amino acids • pharmacokinetics • oxidation • intestinal absorption • dietary protein • dietary carbohydrate • protein metabolism • nitrogen metabolism • sucrose • gastric emptying

This article has been cited by other articles:

				M. Lacroix, C. Bon, C. Bos, J. Leonil, R. Benamouzig, C. Luengo, J. Fauquant, D. Tome, and C. Gaudichon Ultra High Temperature Treatment, but Not Pasteurization, Affects the Postprandial Kinetics of Milk Proteins in Humans J. Nutr., December 1, 2008; 138(12): 2342 - 2347. [Abstract] [Full Text] [PDF]

				F. Mariotti, J. F. Huneau, I. Szezepanski, K. J. Petzke, Y. Aggoun, D. Tome, and D. Bonnet Meal Amino Acids with Varied Levels of Arginine do Not Affect Postprandial Vascular Endothelial Function in Healthy Young Men J. Nutr., June 1, 2007; 137(6): 1383 - 1389. [Abstract] [Full Text] [PDF]

				C. Bos, G. Airinei, F. Mariotti, R. Benamouzig, S. Berot, J. Evrard, E. Fenart, D. Tome, and C. Gaudichon The Poor Digestibility of Rapeseed Protein Is Balanced by Its Very High Metabolic Utilization in Humans J. Nutr., March 1, 2007; 137(3): 594 - 600. [Abstract] [Full Text] [PDF]

				C. Bos, B. Juillet, H. Fouillet, L. Turlan, S. Dare, C. Luengo, R. N'tounda, R. Benamouzig, N. Gausseres, D. Tome, et al. Postprandial metabolic utilization of wheat protein in humans Am. J. Clinical Nutrition, January 1, 2005; 81(1): 87 - 94. [Abstract] [Full Text] [PDF]

				C. Morens, C. Bos, M. E. Pueyo, R. Benamouzig, N. Gausseres, C. Luengo, D. Tome, and C. Gaudichon Increasing Habitual Protein Intake Accentuates Differences in Postprandial Dietary Nitrogen Utilization between Protein Sources in Humans J. Nutr., September 1, 2003; 133(9): 2733 - 2740. [Abstract] [Full Text] [PDF]

				H. Fouillet, C. Gaudichon, C. Bos, F. Mariotti, and D. Tome Contribution of plasma proteins to splanchnic and total anabolic utilization of dietary nitrogen in humans Am J Physiol Endocrinol Metab, July 1, 2003; 285(1): E88 - E97. [Abstract] [Full Text] [PDF]

				C. Bos, C. C. Metges, C. Gaudichon, K. J. Petzke, M. E. Pueyo, C. Morens, J. Everwand, R. Benamouzig, and D. Tome Postprandial Kinetics of Dietary Amino Acids Are the Main Determinant of Their Metabolism after Soy or Milk Protein Ingestion in Humans J. Nutr., May 1, 2003; 133(5): 1308 - 1315. [Abstract] [Full Text] [PDF]

				H. Fouillet, C. Bos, C. Gaudichon, and D. Tome Approaches to Quantifying Protein Metabolism in Response to Nutrient Ingestion J. Nutr., October 1, 2002; 132(10): 3208S - 3218. [Abstract] [Full Text] [PDF]

				H. Fouillet, F. Mariotti, C. Gaudichon, C. Bos, and D. Tome Peripheral and Splanchnic Metabolism of Dietary Nitrogen Are Differently Affected by the Protein Source in Humans as Assessed by Compartmental Modeling J. Nutr., January 1, 2002; 132(1): 125 - 133. [Abstract] [Full Text] [PDF]

				F. Mariotti, M. E Pueyo, D. Tome, R. Benamouzig, and S. Mahe Guar gum does not impair the absorption and utilization of dietary nitrogen but affects early endogenous urea kinetics in humans Am. J. Clinical Nutrition, October 1, 2001; 74(4): 487 - 493. [Abstract] [Full Text] [PDF]

				H. Fouillet, C. Gaudichon, F. Mariotti, C. Bos, J. F. Huneau, and D. Tome Energy nutrients modulate the splanchnic sequestration of dietary nitrogen in humans: a compartmental analysis Am J Physiol Endocrinol Metab, August 1, 2001; 281(2): E248 - E260. [Abstract] [Full Text] [PDF]

				F. Mariotti, M. E Pueyo, D. Tomé, S. Bérot, R. Benamouzig, and S. Mahé The Influence of the Albumin Fraction on the Bioavailability and Postprandial Utilization of Pea Protein Given Selectively to Humans J. Nutr., June 1, 2001; 131(6): 1706 - 1713. [Abstract] [Full Text]