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American Journal of Clinical Nutrition, Vol 49, 490-494, Copyright © 1989 by The American Society for Clinical Nutrition, Inc

ORIGINAL RESEARCH COMMUNICATIONS

Conjunctival impression cytology: feasibility of a field trial to detect subclinical vitamin A deficiency

CL Kjolhede, AM Gadomski, J Wittpenn, J Bulux, AR Rosas, NW Solomons, KH Brown and MR Forman
Department of International Health, Johns Hopkins University, Baltimore, MD.

Two hundred and thirty-six Guatemalan children aged 2-14 y were examined for subclinical vitamin A deficiency by use of conjunctival impression cytology (CIC). The feasibility of the technique and quality of a modified touch procedure were assessed. Neither lid speculum nor physical restraint in the form of a papoose board was used. The CIC technique was easily performed on children aged greater than 3 y but was more difficult among those less than or equal to 3 y. Laboratory processing of specimens is uncomplicated but interpretation may be problematic and requires clearly defined criteria of normal and abnormal cytology as well as careful standardization of readers.


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N. G. Congdon and K. P. West Jr.
Physiologic Indicators of Vitamin A Status
J. Nutr., September 1, 2002; 132(9): 2889S - 2894.
[Abstract] [Full Text] [PDF]


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