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American Journal of Clinical Nutrition, Vol 47, 75-79, Copyright © 1988 by The American Society for Clinical Nutrition, Inc

ORIGINAL RESEARCH COMMUNICATIONS

Folate transport in ileal brush border-membrane vesicles following extensive resection of proximal and middle small intestine in the rat

HM Said, R Redha, W Tipton and W Nylander
Department of Pediatric Gastroenterology, Vanderbilt University School of Medicine, Nashville, TN.

Uptake of folic acid (PteGlu) was examined in remnant ileum of rats after resection of 65% of the small intestine with the brush border- membrane vesicle technique. The results were compared to that of sham- operated rats. In both rat groups transport of PteGlu was linear for approximately 40 s of incubation and was similar in the presence of a Na+ and a K+ gradient (out greater than in). In resected rats transport of PteGlu was inhibited by the structural analogues 5- methyltetrahydrofolate (5-CH3H4PteGlu) and methotrexate (MTX), by sulfasalazine, and by 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) and was saturable as a function of concentration (apparent Kt = 18.3 microM). In the ileum of sham-operated rats, on the other hand, transport of PteGlu was not affected by 5-CH3H4PteGlu, MTX, sulfasalazine, or DIDS and was linear with concentration. These results suggest that the PteGlu transport system is induced in remnant ileum of the rat after extensive intestinal resection.




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Copyright © 1988 by The American Society for Nutrition