AJCN North Carolina Research Campus
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Francis, K. T.
Right arrow Articles by Krumdieck, C. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Francis, K. T.
Right arrow Articles by Krumdieck, C. L.
Agricola
Right arrow Articles by Francis, K. T.
Right arrow Articles by Krumdieck, C. L.

American Journal of Clinical Nutrition, Vol 30, 2028-2032, Copyright © 1977 by The American Society for Clinical Nutrition, Inc

ORIGINAL RESEARCH COMMUNICATIONS

Reaction of tetrahydrofolic acid with cyanate from urea solutions: formation of an inactive folate derivative

KT Francis, RW Thompson and CL Krumdieck

Liver extracts prepared in 6 M urea contain significantly less microbiologically assayable folates than extracts prepared in hot 1% ascorbate. The possibility that inactive folate derivatives are formed by reaction with the cyanate present in the urea solution was investigated. Doubly labeled 2-14C, 3',5',9(n)-3H tetrahydrofolic acid reacted under hydrogen with sodium cyanate gave a compound which, after acidification to remove excess cyanate, shows a single UV absorbing peak at 285 nm. The 14C/3H ratios of starting material and product were the same. Reaction of 14C urea with nonradioactive tetrahydrofolic acid yielded the 14C labeled derivative. The compound does not support Lactobacillus casei growth. It is postulated that folate inactivation in cyanate or thiocyanate treated patients and in uremic patients may have clinical significance.


This article has been cited by other articles:


Home page
 Cancer Epidemiol. Biomarkers Prev.Home page
C. S. Furniss, C. J. Marsit, E. A. Houseman, K. Eddy, and K. T. Kelsey
Line Region Hypomethylation Is Associated with Lifestyle and Differs by Human Papillomavirus Status in Head and Neck Squamous Cell Carcinomas
Cancer Epidemiol. Biomarkers Prev., April 1, 2008; 17(4): 966 - 971.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
C. J. Piyathilake and G. L. Johanning
Cellular Vitamins, DNA Methylation and Cancer Risk
J. Nutr., August 1, 2002; 132(8): 2340S - 2344.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1977 by The American Society for Nutrition