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American Journal of Clinical Nutrition, Vol 25, 273-274, Copyright © 1972 by The American Society for Clinical Nutrition, Inc.

Modified thiochrome procedure for the determination of urinary thiamin

Gilbert A. Leveille Ph.D.1

1 From the Laboratory of Nutritional Biochemistry, Department of Animal Science, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801

A modified procedure is presented for the determination of thiamin in urine avoiding the isolation of thiamin from urine. Blank and standard tubes are used for each sample that correct for native fluorescence in urine as well as for factors appearing to quench thiochrome fluorescence. The modified procedure yields results comparable to those obtained with the widely employed ICNND procedure.






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Copyright © 1972 by The American Society for Nutrition