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American Journal of Clinical Nutrition, Vol 25, 1299-1305, Copyright © 1972 by The American Society for Clinical Nutrition, Inc.

Theoretical basis for anaerobic methodology

David J. Hentges Ph.D.1 and Bruce R. Maier Ph.D.1

1 From the Department of Microbiology, University of Missouri, School of Medicine, Columbia, Missouri

Exposure to air is growth limiting to anaerobes. The exact nature of this growth inhibition is not known, but recent information suggests that exclusion of molecular oxygen from a medium will permit growth of some anaerobes in the presence of a highly oxidized growth environment.

Oxidation-reduction potentials provide a quantitative index of readily available electrons in a liquid medium and reducing conditions are generally correlative to a lack of oxygen. Electrode systems and indicator dyes are means of measuring oxidation-reduction potentials.

Conditions that are important in the growth of anaerobic bacteria must be delineated. Oxygen concentrations and Eh levels that duplicate the in vivo environment are critical in the cultivation of anaerobic bacteria. Future investigations should attempt to elucidate the mechanisms of anaerobiosis.






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Copyright © 1972 by The American Society for Nutrition